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cfx96 touchtm real time fluorescent quantitative pcr instrument  (Bio-Rad)


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    Bio-Rad cfx96 touchtm real time fluorescent quantitative pcr instrument
    Cfx96 Touchtm Real Time Fluorescent Quantitative Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+touchtm+real+time+pcr+instrument/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41900994-158-6-13
    Average 96 stars, based on 27901 article reviews
    cfx96 touchtm real time fluorescent quantitative pcr instrument - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Rumen-protected l-carnitine supplementation during mating period altered metabolic status and reproductive performance of ewes.
    Article Snippet: Funding information Ferdowsi University of Mashhad, Grant/Award Number: 102154; Kaesler Nutrition GmbH Abstract Current study hypothesized that dietary L‐carnitine (LC) inclusion during the mating period ameliorates both metabolic status and reproductive performance of ewes.. Seventy Baluchi ewes (52 ± 4.2 kg of bodyweight and 18 ± 6 months old of age) were enrolled in this study.. Animals were randomly allocated into two dietary treatments, control (only basal diet) or basal diet plus supplementation with a rumen‐protected LC (Carneon 20 Rumin‐pro; 20% LC; Kaesler Nutrition GmbH) at the rate of 10 g/head/ day from 21 days before until 35 days after introducing rams to the ewes (MP).

    Article Title: Lignin reduction in sugarcane by performing CRISPR/Cas9 site-direct mutation of SoLIM transcription factor.
    Article Snippet: .. The amplification procedure included the application of the following conditions: an initial denaturation step at a temperature of 95 ◦C for a period of 30 s. Subsequently, a series of 40 denaturation cycles were conducted, each at a temperature of 94 ◦C for a length of 5 s. This was followed by annealing at a temperature of 58 ◦C for a period of 15 s and extension at a temperature of 72 ◦C for a duration of 10 s. The amplification process was conducted with a CFX96 TouchTM Real-Time PCR instrument manufactured by BIO-RAD®. ..

    Article Title: Cyclooxygenase 2 Promotes Proliferation and Invasion in Ovarian Cancer Cells via the PGE2/NF-κB Pathway
    Article Snippet: Next, 1 μg of total RNA was converted to cDNA using oligo-dT primers and Superscript III reverse transcriptase (Takara, Shiga, Japan). .. Quantitative polymerase chain reaction (qPCR) was performed using an iQTM SYBR® Green Supermix kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA) on a CFX96 TouchTM real-time PCR instrument (Bio-Rad Laboratories, Inc.), using oligonucleotides specific for human COX2 (forward: 5’-TCAAGTCCCTGAGCATCTACGGTT-3’, reverse: 3’-CTGTTGTGTTCCCGCAGCCAGATT-5’), PTGER2 (forward: 5’-GTCTGCTCCTTGCCTTTCAC-3’, reverse: 3’-TGAACGCATTAGTCTCAGAACAG-5’) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (forward: 5’-CTTAGCACCCCTGGCCAAG-3’, reverse: 3’-GATGTTCTGGAGAGCCCCG-5’). ..

    Article Title: Gonadotropins promote human ovarian cancer cell migration and invasion via a cyclooxygenase 2-dependent pathway.
    Article Snippet: The cDNA was amplified by PCR using Premix Ex TaqTM (Takara). .. Quantitative real-time PCR (qRT-PCR) was performed using an iQTM SYBR® Green Supermix kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA) on a CFX96 TouchTM Real-time PCR instrument (Bio-Rad Laboratories, Inc.). ..

    Article Title: Machine learning-based construction of a ferroptosis and necroptosis associated lncRNA signature for predicting prognosis and immunotherapy response in hepatocellular cancer
    Article Snippet: Quantitative analysis was performed using TB Green Premix Ex Taq II (Takara Bio). .. The primers were synthesized by Sangon Biotech Co. Ltd. (Shanghai, China) , Real-time quantitative polymerase chain reaction (qPCR) was performed using a CFX96 TouchTM Real-Time PCR instrument (Bio-Rad, Hercules, CA, USA). ..

    Article Title: The effect of modification of DNA interference on myostatin gene expression in mice
    Article Snippet: Myostatin is a known negative regulator of muscle tissue growth.. Thus, an inhibitor of myostatin may be therapeutically useful as an anabolic agent for the muscle tissue.. A promising gene-silencing approach for gene therapy is DNA interference (DNAi), a sequence that is complementary to the promoter region of a target gene.

    Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury
    Article Snippet: Real-time PCR (qRT-PCR) was performed on cDNA prepared from 2 μg of total RNA using (Thermo Fisher Scientific, San Diego, CA, USA) reverse transcriptase according to manufacturer’s directions (iScript, #1708891, Biorad, Hercules, CA). .. Gene expression was measured using SYBR ® Green (# 1725271, Biorad) on a CFX96 TouchTM Real-Time PCR Instrument with Quantitect ® primers from Qiagen (Venlo, The Netherlands) specific for the following genes: Rab5a (QT00171969), Rab35 (QT00107268), Vps4a (QT00145089), and Tsg101 (QT00143192). qRT-PCR was performed with the following parameters: a preamplification at 95 °C for three min, 40 cycles of 95 °C for 10 s, 55 °C for 10 s, 72 °C for 30 s for a melt curve analysis. .. All processes were performed in duplicate, and analyzed by CFX manager software (Version 3.1, Biorad, Hercules, CA, USA).

    Article Title: No evidence for interactions of dimethylfumarate (DMF) and its main metabolite monomethylfumarate (MMF) with human cytochrome P450 (CYP) enzymes and the P‐glycoprotein (P‐gp) drug transporter
    Article Snippet: .. Resultant cDNA was diluted 1:5 with nuclease‐free water before analysis by RT‐qPCR performed in a CFX96 TouchTM real‐time PCR instrument (Bio‐Rad Laboratories). .. The 96‐well plates and SsoAdvancedTM Universal SYBR ® Green Supermix (Bio‐Rad Laboratories) were used according to the manufacturer's instructions.

    Amplification:

    Article Title: Lignin reduction in sugarcane by performing CRISPR/Cas9 site-direct mutation of SoLIM transcription factor.
    Article Snippet: .. The amplification procedure included the application of the following conditions: an initial denaturation step at a temperature of 95 ◦C for a period of 30 s. Subsequently, a series of 40 denaturation cycles were conducted, each at a temperature of 94 ◦C for a length of 5 s. This was followed by annealing at a temperature of 58 ◦C for a period of 15 s and extension at a temperature of 72 ◦C for a duration of 10 s. The amplification process was conducted with a CFX96 TouchTM Real-Time PCR instrument manufactured by BIO-RAD®. ..

    SYBR Green Assay:

    Article Title: Cyclooxygenase 2 Promotes Proliferation and Invasion in Ovarian Cancer Cells via the PGE2/NF-κB Pathway
    Article Snippet: Next, 1 μg of total RNA was converted to cDNA using oligo-dT primers and Superscript III reverse transcriptase (Takara, Shiga, Japan). .. Quantitative polymerase chain reaction (qPCR) was performed using an iQTM SYBR® Green Supermix kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA) on a CFX96 TouchTM real-time PCR instrument (Bio-Rad Laboratories, Inc.), using oligonucleotides specific for human COX2 (forward: 5’-TCAAGTCCCTGAGCATCTACGGTT-3’, reverse: 3’-CTGTTGTGTTCCCGCAGCCAGATT-5’), PTGER2 (forward: 5’-GTCTGCTCCTTGCCTTTCAC-3’, reverse: 3’-TGAACGCATTAGTCTCAGAACAG-5’) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (forward: 5’-CTTAGCACCCCTGGCCAAG-3’, reverse: 3’-GATGTTCTGGAGAGCCCCG-5’). ..

    Article Title: Gonadotropins promote human ovarian cancer cell migration and invasion via a cyclooxygenase 2-dependent pathway.
    Article Snippet: The cDNA was amplified by PCR using Premix Ex TaqTM (Takara). .. Quantitative real-time PCR (qRT-PCR) was performed using an iQTM SYBR® Green Supermix kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA) on a CFX96 TouchTM Real-time PCR instrument (Bio-Rad Laboratories, Inc.). ..

    Article Title: The effect of modification of DNA interference on myostatin gene expression in mice
    Article Snippet: Myostatin is a known negative regulator of muscle tissue growth.. Thus, an inhibitor of myostatin may be therapeutically useful as an anabolic agent for the muscle tissue.. A promising gene-silencing approach for gene therapy is DNA interference (DNAi), a sequence that is complementary to the promoter region of a target gene.

    Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury
    Article Snippet: Real-time PCR (qRT-PCR) was performed on cDNA prepared from 2 μg of total RNA using (Thermo Fisher Scientific, San Diego, CA, USA) reverse transcriptase according to manufacturer’s directions (iScript, #1708891, Biorad, Hercules, CA). .. Gene expression was measured using SYBR ® Green (# 1725271, Biorad) on a CFX96 TouchTM Real-Time PCR Instrument with Quantitect ® primers from Qiagen (Venlo, The Netherlands) specific for the following genes: Rab5a (QT00171969), Rab35 (QT00107268), Vps4a (QT00145089), and Tsg101 (QT00143192). qRT-PCR was performed with the following parameters: a preamplification at 95 °C for three min, 40 cycles of 95 °C for 10 s, 55 °C for 10 s, 72 °C for 30 s for a melt curve analysis. .. All processes were performed in duplicate, and analyzed by CFX manager software (Version 3.1, Biorad, Hercules, CA, USA).

    Synthesized:

    Article Title: Machine learning-based construction of a ferroptosis and necroptosis associated lncRNA signature for predicting prognosis and immunotherapy response in hepatocellular cancer
    Article Snippet: Quantitative analysis was performed using TB Green Premix Ex Taq II (Takara Bio). .. The primers were synthesized by Sangon Biotech Co. Ltd. (Shanghai, China) , Real-time quantitative polymerase chain reaction (qPCR) was performed using a CFX96 TouchTM Real-Time PCR instrument (Bio-Rad, Hercules, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: The effect of modification of DNA interference on myostatin gene expression in mice
    Article Snippet: Myostatin is a known negative regulator of muscle tissue growth.. Thus, an inhibitor of myostatin may be therapeutically useful as an anabolic agent for the muscle tissue.. A promising gene-silencing approach for gene therapy is DNA interference (DNAi), a sequence that is complementary to the promoter region of a target gene.

    Gene Expression:

    Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury
    Article Snippet: Real-time PCR (qRT-PCR) was performed on cDNA prepared from 2 μg of total RNA using (Thermo Fisher Scientific, San Diego, CA, USA) reverse transcriptase according to manufacturer’s directions (iScript, #1708891, Biorad, Hercules, CA). .. Gene expression was measured using SYBR ® Green (# 1725271, Biorad) on a CFX96 TouchTM Real-Time PCR Instrument with Quantitect ® primers from Qiagen (Venlo, The Netherlands) specific for the following genes: Rab5a (QT00171969), Rab35 (QT00107268), Vps4a (QT00145089), and Tsg101 (QT00143192). qRT-PCR was performed with the following parameters: a preamplification at 95 °C for three min, 40 cycles of 95 °C for 10 s, 55 °C for 10 s, 72 °C for 30 s for a melt curve analysis. .. All processes were performed in duplicate, and analyzed by CFX manager software (Version 3.1, Biorad, Hercules, CA, USA).

    Quantitative RT-PCR:

    Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury
    Article Snippet: Real-time PCR (qRT-PCR) was performed on cDNA prepared from 2 μg of total RNA using (Thermo Fisher Scientific, San Diego, CA, USA) reverse transcriptase according to manufacturer’s directions (iScript, #1708891, Biorad, Hercules, CA). .. Gene expression was measured using SYBR ® Green (# 1725271, Biorad) on a CFX96 TouchTM Real-Time PCR Instrument with Quantitect ® primers from Qiagen (Venlo, The Netherlands) specific for the following genes: Rab5a (QT00171969), Rab35 (QT00107268), Vps4a (QT00145089), and Tsg101 (QT00143192). qRT-PCR was performed with the following parameters: a preamplification at 95 °C for three min, 40 cycles of 95 °C for 10 s, 55 °C for 10 s, 72 °C for 30 s for a melt curve analysis. .. All processes were performed in duplicate, and analyzed by CFX manager software (Version 3.1, Biorad, Hercules, CA, USA).



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